KLF 4 ( Kruppel - like factor 4 ( gut ) )

A full-length mouse cDNA clone encoding KLF4 was initially isolated from a NIH3T3 cDNA library by reduced stringency screening with a DNA probe containing the zinc finger region of an immediate early gene product, Zif268 or Egr1. A distinct feature of the KLF4 gene is the highly GC-rich nature of the sequence near its 5'-end. Thus, the G+C content of the 1000 nt 5'-flanking region is 67% and that of the 5'untranslated region is 63%. Moreover, the bulk of the GC residues are concentrated in the region between nt 600 and +300 of the gene where the G+C content is 82%. The gene has four exons, each containing a portion of the translated region.